Isolated Nuclei DNA Staining

Nuclei for Cell Staining Analysis

Hypotonic Staining Buffer:

1. Aliquot 1x10⁶ cells into each tube.
2. Centrifuge 200 to 400 xg for 5 - 10 minutes and aspirate supernatant without disturbing the pellet.
3. Add 500 ul of the Hypotonic Staining Buffer to the pellet and gently mix well.
4. Keep samples at 4°C protected from light for 30 min or for a maximum of 1 h before acquisition on the flow cytometer.

Note:

Debris can incease with longer staining incubation times.

Reference

Krishan A: Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining. J. Cell Biol. 66:188-193, 1975.