Histology and Immunohistochemistry Shared Resource
Leader
Nissi M. Varki, MD
Professor of Medicine and Pathology
Phone: (858) 534-4933; e-mail: nvarki@ucsd.edu
Contact
Laarni Gapuz, MT (ASCP)
Phone: (858) 822-1011, e-mail: lgapuz@ucsd.edu
Location: Moores Cancer Center, 3855 Health Sciences Drive #0815, #5314 (fifth floor)
Alternate:
MaryAnn Lawrence, HT (ASCP)
Histology Lab Manager
Phone: 858-534-2544, e-mail mlawrence@ucsd.edu
Location: Center for Molecular Medicine/East, Room 1080, 9500 Gilman Drive
Service profile
- Freezing and embedding of tissues for histology
- Frozen sections
- Paraffin blocks processing and Paraffin sections
- Hematoxylin and Eosin staining of sections
- Specialized histochemical staining
- Individualized immunohistochemical assays
- Specialized assays: TUNEL, BrdU, beta galactosidase, etc.
- Interpretation assistance; mouse histology and pathology
- Design and interpretation of immunohistology and in situ hybridization analysis
- Laser Capture Microdissection
Overview
This facility allows a complete histologic phenotypic analysis of tissues from genetically altered mice; embryo to adult. It is indispensable for providing quality control for the presence of cancer in a human tissue sample prior to use in biochemical or molecular biological analysis. Conversely, it establishes the absence of tumor in tissue utilized as a normal control for similar experiments.
Important information
Powerpoint presentations
(Please allow a reasonable amount of time to load)
Graphic example of how genetically altered animals can be analyzed optimally
(Click image for full size)
