Website:

http://www.cancer.ucsd.edu/tgm   
   

Leader:

Pamela Mellon, Ph.D.
Professor of Reproducive Medicine and Neuroscience
(858) 534-1140 / mellonadmin@ucsd.edu  

Contact:

Name: Ella Kothari
stemc@ad.ucsd.edu

Service Profile:

- Pronuclear Microinjection of DNA to Create Transgenic Mice

- Blastocyst Microinjection of Embryonic Stem Cells to Create Knockout Mice

- Embryonic Stem Cell Gene Targeting for Homologous Recombination

- Ovary Transplantation to Rescue Infertile Mice

- Embryo Rederivation to Generate Pathogen-Free Mice

Overview of the Shared Resource:

The Transgenic Core and Embryonic Stem Cell Shared Resource is a state-of-the-art facility that has an outstanding track record in the production of genetically altered mice. Transgenic mice carrying new or novel genes are created by microinjection of DNA into the pronuclei of fertilized eggs. Knock-out" mice lacking specific genes of interest are created by homologous recombination in embryonic stem cells followed by injection into blastocysts to create chimeric mice. Highly experienced personnel produce transgenic and knock-out mice for UCSD investigators at very reasonable cost and with very short lead times.

A research service of the NCI-designated Moores UCSD Cancer Center: http://www.cancer.ucsd.edu