SHARED RESOURCES - HISTOLOGY & IMMUNOHISTOCHEMISTRY

Website: http:// www.cancer.ucsd.edu/histology Leader: Nissi M. Varki, MD Professor of Medicine and Pathology 858-534-4933 / nvarki@ucsd.edu Contact: Laarni Gapuz, MT (ASCP) email: lgapuz@ucsd.edu Phone: (858) 822-1011 Location: Moores Cancer Center, 3855 Health Sciences Drive #0815, Fifth floor #5314

Service Profile:

- Freezing and embedding of tissues for histology

- Frozen sections

- Paraffin blocks processing and Paraffin sections

- Hematoxylin and Eosin staining of sections

- Specialized histochemical staining

- Individualized immunohistochemical assays

- Specialized assays: TUNEL, BrdU, beta galactosidase, etc.

- Interpretation assistance; mouse histology and pathology
- Design and interpretation of immunohistology and in situ hybridization analysis

- Laser Capture Microdissection

 

Overview of the Shared Resource:

This facility allows a complete histologic phenotypic analysis of tissues from genetically altered mice; embryo to adult. It is indispensable for providing quality control for the presence of cancer in a human tissue sample prior to use in biochemical or molecular biological analysis. Conversely, it establishes the absence of tumor in tissue utilized as a normal control for similar experiments.

A research service of the NCI-designated Moores UCSD Cancer Center: http://www.cancer.ucsd.edu