Cancer Imaging Network (CIN)

Human Breast Cancer Cells in Living Zebrafish Mixed MDA CFP parental (blue) and MDA RhoC DsRed (red) (1:1 ratio) human breast cancer cells growing in Tg( fli :EGFP) zebrafish (green blood vessels), 6 days post injection, were imaged with a Nikon C1si confocal microscope (60x magnification). 3-D reconstruction was made with Imaris software using the isosurfacing module.

Overview:
Researchers at the UC San Diego School of Medicine's Pathology Department and the Moores Cancer Center are utilizing computer-assisted, spectral confocal and multiphoton microscopy to visualize cancer progression and tumor-induced angiogenesis. This initiative brings together a network of clinical and basic science investigators with a common goal – to enhance our understanding of human cancer progression through computer-assisted, 3-D imaging.  The collaborative effort provides cancer researchers the unique ability to: 

• Generate quantitative high resolution, 3-D reconstructions of developing tumors and their supporting vascular network. 

• Track invasive cancer cells in vitro and in vivo. 

• Perform 3-D time-lapse measurements of dynamic cancer formation and angiogenesis in live animal and cell culture systems.  

For more information on software capabilities and availability, contact us or visit
      www.bitplane.com (Imaris)
      http://www.moleculardevices.com/pages/software/metamorph.html (MetaMorph)
      http://www.improvision.com/products/volocity/visualization/ (Volocity)

Graphics System Profile:

• Imaris Suite 4.5, including:
        Filament Tracer 4.5
        AutoAligner 2.0 Irix or Win
        AutoDeblur X1 Gold Confocal & Widefield
        ImarisXT 4.5
• MetaMorph Premier Offline, Version 7
• Volocity Visualization Software
• Alienware Graphics Workstation, featuring:
        AMD Athlon 64 FX-60 with HyperTransport and Dual Core Technology processor
        Quad NVIDIA SLI graphics processor featuring four GeForce 7900 GPUs
        AlienAdrenaline video performance optimizer
        4GB Low Latency DDR PC-3200 SDRAM
        32” Samsung SyncMaster LCD monitor

 Contact:

Name: Richard L. Klemke, Ph.D.
Phone: (858) 822-5610
Fax: (858) 822-4566
Email: rklemke@ucsd.edu
Location: La Jolla Campus, Basic Science Building, Room 1040A
Mailcode: 0612