Research & Training

Microscopy

Leader

James R. Feramisco, PhD
Professor of Medicine

  • E-mail: jferamisco@ucsd.edu
  • Phone: (858) 534-7287
  • Fax: (858) 534-1422
  • Location: La Jolla Campus - Cellular & Molecular Medicine Building, East, Lower Floor

Service profile

  • Conventional, Confocal and Deconvolution Light Microscopy
  • Image Analysis and Photoproduction
  • Fluorescence In Situ Hybridization and Chromosomal Painting
  • Cell Preparation and Fluorescence Staining
  • 3-D Digital Image Capture (still and time lapse) and Analysis
  • Training & Consultation
  • Enhanced Tissue Imaging
  • Access to Needle Microinjection Equipment
  • Enhanced 3-D Perspective Views in Collaboration with SDSC

Equipment profile

  • Delta Vision Deconvolution Microscope System (Nikon TE-200 Microscope)
  • Hamamatsu Cooled CCD Video-Camera connected to a Zeiss Axiophot
  • Zeiss LSM510 Laser Scanning Confocal Microscope
  • Xenogen IVIS 200 system for live animal bioluminescence and fluorescence imaging
  • Nikon TE300 inverted fluorescence microscope with z-stepping motor
  • Nikon E600 upright fluorescence microscope with SPOT RT color camera
  • Eppendorf Semi-Automatic Cell Microinjector
  • Unix PC and Macintosh Graphics Workstations
  • Software Packages & Color Printers
  • iCys Research Imaging Cytometer

Overview

Provides a group of specialized techniques and rare equipment to examine cellular functions of candidate genes. Offers expensive and powerful image-capture technology, in lapsed or real time, which can be critical to achieve basic research objectives of today's laboratory. Interfaces with the VisLab of the San Diego Supercomputer Center to provide enhanced 3-D perspective views of data sets. The DeltaVision and Zeiss LSCM are housed in the new SOM Microscopy Facility along with the Neurosciences Microscopy Core.

For more information, see:

Contacts and facility access

For access to the above equipment, contact Kersi Pestonjamasp at (858) 822-3895, (858) 534-8803, or kpestonjamasp@ucsd.edu, or Steve McMullen at (858) 822-4187 or swmcmullen@ucsd.edu.

For IVIS 200, contact Joseph Aguilera at (858) 822-0253 or jaguilera@ucsd.edu.

For creating enhanced 3-D perspective views using MeshViewer, Volume Explorer, and other visualization tools offered by the VisLab, contact Colin McGinn at cmcginn@ucsd.edu.

For access to the iCys Research Imaging Cytometer, contact Joseph Aguilera at (858) 822-4180, jaquilera@ucsd.edu

Locations

  • Cellular & Molecular Medicine East, Basement
  • DeltaVision deconvolution system & Zeiss LSM510 confocal
  • Moores Cancer Center Vivarium - IVIS 200
  • Moores Cancer Center, Room 2304 - Nikon upright and teaching microscopes
  • Moores Cancer Center, Room 5317 - Nikon inverted microscope

Online calendar

http://microscopy.ucsd.edu/schedule